CD317 (BST2) Mouse anti-Human, Rhesus, Baboon, Cynomolgus, Brilliant Violet 421, Clone: Y129 (also known as HM1.24), BD

Description

• The Y129 monoclonal antibody specifically binds to CD317 which is also known as Bone marrow stromal antigen 2 (BST2)
• CD317 is a type II transmembrane glycoprotein that belongs to the Tetherin family
• CD317 is an interferon (IFN)-induced protein that is expressed on monocytes, granulocytes, dendritic cells, B lymphoblasts, plasma cells, T cells, natural killer (NK) cells, stromal cells and myeloma cells
• CD317 is expressed by nonhematopoietic cells including cells within solid tumors derived from breast, lung and kidney
• CD317 may be involved in the interactions between bone marrow stromal cells and lymphocytes
• It is likewise known as Tetherin and reportedly blocks the release of some enveloped viruses by tethering virions to infected cell membranes
• CD317 stimulates signaling by CD85g (ILT7) and may provide negative feedback for interferon (IFN) production by plasmacytoid dendritic cells during viral infections
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.