Cox-2 Mouse, Unlabeled, Clone: 33, BD

Description

• Cyclooxygenase (Cox) is also known as prostaglandin H synthase or PGH synthase (E.C
• 1.14.99.1)
• It catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), the precursor of PGE2, PGF2α, PGD2, prostacyclin, and thromboxane A2
• Cox actually has two different enzymatic activities: a cyclooxygenase that mediates the formation of PGG2 from oxygen and arachidonate and a hydroperoxidase that catalyzes a reduction of PGG2 yielding PGH2
• Two Cox genes, Cox-1 and Cox-2, have been isolated in several species
• A 4kb mRNA encodes the 604 amino acid Cox-2 protein
• The two human Cox isoenzymes are 61% identical in amino acid composition with the active sites being highly conserved
• Cox-2 mRNA and protein levels are induced by serum, lipopolysaccharides, growth factors, human chorionic gonadotropin and phorbol testers in various mammalian cell types
• It has been shown that interleukin-1α (IL-1α) induces increased levels of Cox-2 mRNA and protein in human endothelial cells
• The sustained increase in Cox-2 is apparently due (at least in part) to IL-1α increasing the stability of Cox-2 mRNA
• This type of regulatory mechanism may play an important role in chronic inflammatory conditions
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting