Cadherin-5 Mouse anti-Human, Unlabeled, Clone: 75, BD

Description

• Cadherins are a family of transmembrane glycoproteins involved in the Ca 2 + - dependent cell-cell adhesion that occurs in many tissues
• These proteins are similar in their domain structure (45-74% amino acid conservation), Ca 2 + and protease-sensitivity, and molecular weight
• Cadherin-5 (VE-Cadherin or CD144) is one of a number of cadherins (cadherin-4 through -11) whose cDNAs were isolated from rat brain and retina
• These cadherins have a cytoplasmic domain that is highly conserved relative to previously identified cadherins, indicating that this domain is essential for cell adhesion activity
• This function is mediated by cadherin interaction with cytoskeletal proteins
• However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins
• Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells
• A new type of adhering junction has been identified in certain vascular endothelial cells
• These junctions are known as “complexus adherens” and are morphologically and compositionally distinct from desmosomes and zonula adherens junctions
• The complexus adherens of endothelial cells lack desmosomal cadherins as well as E-Cadherin
• However, these cells are rich in Cadherin-5 which colocalizes with desmoplakin and γ-Catenin (plakoglobin)
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting