p47[phox] Mouse anti-Human, Unlabeled, Clone: 1, BD

Description

• The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli
• The enzyme is dormant in resting neutrophils
• The active site of this enzyme is located in an integral membrane cytochrome, b558, which consists of the two subunits gp91[phox] and p21[phox]
• Superoxide production depends on the formation of a complex that includes two cytosolic proteins, p67[phox] and p47[phox]
• The GTP-binding protein Rac is also an essential component for oxidase activity
• p47[phox] is a highly basic protein that contains two SH3 domains
• The C-terminal quarter of the molecule contains many potential phosphorylation sites, consisting of serines and basic residues
• Expression of p47[phox] is restricted to cells of phagocytic or lymphocytic lineage
• IFN-γ is a potent inducer of both p47[phox] mRNA and protein
• p47[phox] is an early reactant in oxidase assembly and this assembly can be inhibited by a C-terminal peptide of the large subunit of cytochrome b558
• It is thought that p47[phox] binds directly to the cytochrome, while p67[phox] associates with the cytochrome by binding p47[phox]
• Immunofluorescence, Western Blotting