MEF2D Mouse, Unlabeled, Clone: 9, BD

Description

• MEF2D is a member of the MEF2 (Myocyte-specific Enhancer-binding Factor 2) transcription factor family
• Members of this family (MEF2A, B, C, & D) contain several characteristic motifs that include a MEF2 domain and a MADS box
• MEF2 proteins bind the DNA sequence, 5′- (C/T)T(A/T)(A/T)AAATA(A/G)-3′, and binding occurs primarily, although not exclusively, in muscle cells
• MEF2D gene expression is ubiquitous and the mRNA is alternatively spliced
• This results in multiple protein isoforms that heterodimerize with other family members
• In addition to the MEF2 and MADS domains, MEF2D has a glutamine-/proline-rich region, amino acids 365-404, and a basic region, in the MADS domain near the N-terminus
• The glutamine-/proline-rich region is involved in transactivation, while the basic region mediates sequence-specific DNA binding
• MEF2D displays 75% similarity to hMEF2A and hMEF2C
• Myogenin gene transcription requires MEF2D, indicating that MEF2D is involved in myogenesis and muscle cell differentiation
• MEF2D has been reported to have a calculated molecular weight of 56kDa, but can be observed migrating in a range of 56-70kDa
• Immunofluorescence, Immunoprecipitation, Western Blotting