GM130 Mouse, Unlabeled, Clone: 35/GM130, BD

Description

• Maturation and post-translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus
• The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus
• The detergent insoluble fraction of the Golgi is named “matrix” and is required for proper morphology of the Golgi membranes
• GM130 (Golgi matrix protein of 130kDa) is a protein isolated from the Triton™ X-100-insoluble Golgi matrix and peripherally associated with the cis compartment, as demonstrated by co-localization with syntaxin5
• GM130 is homologous to the Golgi autoantigen golgin 95
• GM130 interacts through its N-terminal domain with p115 and with the Golgi membranes at the C-terminal portion
• Furthermore, the mitotic phosphorylation of GM130 blocks the interaction with p115
• Thus, GM130 appears to function as a structural element of the Golgi apparatus that also provides attachment sites for membranes and other Golgi proteins
• The 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status
• Host Species: Mouse Clone: 35 Isotype: IgG1 Species Reactivity [for Features Main]: Human Immunogen: Rat GM130 aa
• 869-982 Immunofluorescence, Immunoprecipitation