p62 Ick ligand Mouse anti-Human, Unlabeled, Clone: 3, BD

Description

• p62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein
• The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites
• Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck
• However, mutation of the tyrosine did not have an effect
• In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCζ catalytic domain
• In turn, PKCζ phosphorylates p62
• p62 lck ligand binds to the dimerization region of PKCζ, thereby inhibiting PKCζ-PKCζ interaction
• This suggests that p62 lck ligand may compete with PKCζ
• However, it requires PKCζ for proper subcellular localization
• These data suggest that p62 lck ligand may be part of the protein bridge that links PKCζ to the tyrosine kinases involved in signaling pathways
• Immunofluorescence, Immunoprecipitation, Western Blotting