PKBα/Akt Mouse, Unlabeled, Clone: 7, BD

Description

• Rac (Related to the A and C kinases) protein kinase shows 73% and 68% to Protein Kinase C and PKA, respectively, and is also known as Protein Kinase B (PKB)
• It was also identified as the product of the v-akt oncogene from T-cell lymphomas
• PKB mRNA is expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary
• Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1
• PKB is activated in response to growth factors through the activation of PI3-kinase and Ras
• Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of PKB coincident with its phosphorylation and activation
• Cellular stress leading to the activation of the p38 MAP kinase also induces PKB activation, indicating a multiplicity of signaling pathways that activate PKB
• Upon activation, PKB associates with some members of the PKC family of kinases, such as PKCδ and PKCζ
• Downstream of PKB, GSK3 is thus far the only identified substrate for PKB phosphorylated in vivo
• Immunofluorescence, Immunoprecipitation, Western Blotting