CIP4 Mouse, Unlabeled, Clone: 21, BD

Description

• Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes
• They cycle between active GTP-bound and inactive GDP-bound states
• CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta
• It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae
• Similarly, CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis
• CDC42-interacting protein 4 (CIP4) was identified in a yeast-two hybrid screen for proteins that bind CDC42
• Another variant of CIP4, CIP4/2, was identified that contains an extra 56 amino acids and has 71% identity with CIP4 (or CIP4/1)
• CIP4 contains a C-terminal SH3 domain and an N-terminal domain that is homologous to non-catalyitic motifs in the tyrosine kinase Fer
• The mRNA expression of CIP4 is highest in skeletal muscle, heart, and placenta
• Overexpression of CIP4 in Swiss 3T3 cells reduces the amount of stress fibers and leads to clustering of CIP4 to foci at the dorsal side of the cells
• In addition, CIP4 binds the Rho-GTPase activating protein RICH and the cytoskeletal protein WASP
• Coexpression of CIP4 and WASP in Cos-7 cells leads to WASP association with microtubules
• Thus, CIP4 is involved in various protein-protein interactions associated with cytoskeletal dynamics
• Immunofluorescence, Western Blotting