Stat1 (pY701) Mouse, PE, Clone: 4A, BD

Description

• Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors
• Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation
• Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes
• Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner
• Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor
• Stat1 has two alternatively spliced isoforms, 91kDa Stat1α and 84kDa Stat1β; Stat1α has 38 additional C-terminal amino acids
• In response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine phosphorylated at Y701, and the complex is translocated to the nucleus
• This results in the formation of an active complex that includes the DNA-binding p48 subunit
• This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs)
• The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1α and Stat1β
• Flow Cytometry, Intracellular Staining