Stat6 (pY641) Mouse anti-Human, Alexa Fluor 488, Clone: 18/P-STAT6, BD

Description

• Interleukin-4 (IL-4), a major immunoregulatory cytokine, is secreted by activated T lymphocytes, basophils, and mast cells and plays an important role in modulating T helper cell lineage development
• It induces specific gene expression via the tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641)
• Stat6, a member of the signal transducers and activators of transcription protein family, mediates signals for IL-4 and, possibly, IL-13
• While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta
• Following cytokine receptor ligation, Jak kinases are activated and phosphorylate the cytoplasmic tails of the oligomerized receptors
• The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain
• Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP
• Thus, Stat6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity
• Flow Cytometry, Intracellular Staining