hSlu7 Mouse anti-Human, Unlabeled, Clone: 35, BD

Description

• Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps
• The first step involves cleavage of the 5 ft
• exon and the production of a lariat intermediate
• In the second step, the 3 ft
• splice site is cleaved and the exons are fused with concomitant release of the intron lariat
• The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors
• Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step
• Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), Prp18p (hPrp18) and Slu7 (hSlu7)
• hSlu7 contains a zinc knuckle motif similar to the yeast Slu7
• This domain is present in retroviral nucleocapsid proteins and in several splicing factors
• hSlu7 associates with the spliceosome late in the splicing pathway during recognition of the 3 ft
• splice site
• During step II of 3 ft
• splicing, hSlu7 tightly binds to exon 1 in the spliceosome and helps specify attack on the correct adenine-guanine dinucleotide, located 18 to 40 nucleotides downstream of the branch site
• Applications: Immunofluorescence, Western Blotting