ICBP90 Mouse anti-Human, Unlabeled, Clone: 28, BD

Description

• DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA
• Topoisomerase I produces transient single-stranded breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA
• These DNA breaks are important for DNA replication and chromosome condensation and segregation
• Two types of topoisomerase II have been identified, TopoIIα and TopoIIβ
• During interphase, TopoIIα is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIα is found associated with metaphase chromosomes and centromeres
• Transcriptional control of TopoIIα expression is regulated through multiple gene promoters, which include five inverted CCAAT boxes, one ATF binding sequence, and two GC-rich boxes
• ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted CCAAT box
• ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow
• Overexpression of ICBP90 in COS-1-transfected cells leads to enhanced expression of TopoIIα
• ICBP90 co-localizes with TopoIIα in proliferating areas of appendix and inhigh grade breast carcinomas
• Thus, ICBP90 may be an important transcripitonal regulator of TopoIIα expression in proliferating cells
• Western Blotting