DRBP76 Mouse anti-Human, Unlabeled, Clone: 21, BD

Description

• The double stranded RNA (dsRNA)-dependent, Ser/Thr protein kinase, PKR, is encoded by an IFN-inducible gene and is critical for the anti-viral responses mediated by IFN
• Interaction with activators such as heparin, dsRNA, and the dsRNA-binding proteins (DRBPs), DRBP76, PACT, and RAX, induces PKR autophosphorylation and activation
• DRBP76 was identified through its binding to dsRNA and PKR
• In addition, DRBP76 has been identified as the alternatively spliced nuclear phosphoproteins of 90kDa (NFAR-1/NF90) and 110kDa (NFAR-2), as well as M-phase phosphoprotein (MPP4), translational control protein 80 (TCP80), and interleukin enhancer binding factor 3 (ILF3)
• DRBP76 contains a bi-partite nuclear localization signal at amino acids 369-373 and 386-394, two DRB domains in the C-terminal half, and a C-terminal RG2 domain that is present in many RNA binding proteins
• DRBP76 binds pre-mRNAs and spliced mRNAs, co-localizes with PKR in the nucleus, and is phosphorylated by PKR and possibly cyclin-dependent kinases
• Thus, DRBP76 may be one of multiple DRBP variants, which associate with PKR and regulate RNA translation
• Immunofluorescence, Western Blotting