MAD2B/Rev7 Mouse, Unlabeled, Clone: 14, BD

Description

• Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins
• These events are of utmost importance at the checkpoints, or transition points, of the cell cycle
• MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis
• Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells
• Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2
• MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis
• This inhibition occurs through binding and suppression of CDC20 activation of APC
• MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC
• Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase ζ subunit Rev3
• Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression
• Immunofluorescence, Western Blotting