Rab4 Mouse, Unlabeled, Clone: 7, BD

Description

• The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking
• The Rab proteins cycle between active GTP-bound and inactive GDP-bound states
• Rab4 is associated with early endosomes and recycling vesicles
• It is involved in the recycling of receptors to the plasma membrane and regulates membrane recycling from early endosomes
• Rab4 has been reported to be membrane-bound during interphase
• However, it can be found as a cytosolic protein during mitosis
• It is thought that Rab4 is phosphorylated by cdc2/cyclin B during mitosis and that Ser196 on Rab4 is the recognition site of cdc2
• Mutation of Ser196 results in Rab4 membrane association during mitosis in an unphosphorylated form
• Together this data indicates that Rab4 is phosphorylated by cdc2 on Ser196 which releases it into the cytosol during mitosis
• In addition, Rab4 may play a role in insulin-induced glucose transport into rat adipocytes
• Rab4 has also been reported to be associated with vesicles that contain GLUT4, a glucose transporter isoform
• Immunofluorescence, Western Blotting