Rab8 Mouse, Unlabeled, Clone: 4, BD

Description

• The members of the Ras superfamily of GTPases, which now includes more than 50 different proteins, shuttle between the GDP-bound inactive position and the GTP-bound active state
• The GTPase activity of the Ras-like proteins is regulated by effectors that promote either the GDP- or the GTP-bound states
• GTPases of the Ras superfamily have a role in controlling numerous events such as cell proliferation, nucleocytoplasmic transport, and vesicular traffic
• The Rab superfamily of proteins includes more than 30 different members that are involved in the regulation of different steps of vesicular traffic
• Subcellular localization and specific cellular function of each Rab appears to be encoded in the unique C terminal part of the proteins, known as the hypervariable region
• The 207 amino acid Rab8 protein is closely related to the yeast Sec4p and regulates vesicle transport from the Trans-golgi Network to the basolateral plasma membrane in epithelial cells
• In addition, Rab8 interacts with the stress-activated kinase rab8p, a close homologue of the GC Kinase
• This interaction was found to be dependent on GTP
• Immunofluorescence, Immunoprecipitation, Western Blotting