Hip1R Mouse, Unlabeled, Clone: 44, BD

Description

• Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis
• Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192kDa each) and three light chains (23-25kDa each)
• A variety of proteins facilitate receptor-mediated endocytosis through association with clathrin-coated vesicles
• Huntingtin interacting protein 1 (Hip1) is an actin-binding protein that interacts with Huntingtin protein, and has been implicated in vesicular transport defects found in Huntingtin's disease
• Hip1 related protein (Hip1R) is another actin binding protein that contains an epsin NH2-terminal homology (ENTH)domain, three coiled-coil regions, a leucine zipper, and a talin-like actin binding domain
• Hip1R mRNA is widely expressed, and Hip1R protein is enriched in the cell cortex and perinuclear region
• The ENTH domain of Hip1R is required for binding to phosphatidylinositol-4,5-bisphosphate, and this complex is essential for clathrin-mediated endocytosis
• In addition, Hip1R colocalizes with clathrin, AP-2 and endocytosed transferrin
• Thus, Hip1R may facilitate interactions between clathrin-coated pits and actin during endocytosis
• Western Blotting