Transferrin Receptor Mouse anti-Human, Unlabeled, Clone: 2, BD

Description

• Iron acquisition is a prerequisite for cell proliferation
• The transferrin receptor is the primary mode of iron uptake
• This receptor mediates iron uptake through internalization and recycling of the iron carrying serum protein transferrin
• Binding of transferrin to its receptor causes rapid internalization into an acidic intracellular compartment where iron dissociates from transferrin
• Both the receptor and transferrin are then recycled through the exocytic pathway
• The transferrin receptor is expressed in most cells and tissues, especially proliferating cells, and the receptor is expressed as a dimer of identical integral membrane proteins
• Transferrin receptor mRNA expression is inversely proportional to iron availability, and this regulation occurs through post-transcriptional control of mRNA stability
• Transferrin receptor mRNA contains iron responsive elements (IRE) that are bound by IRE-binding proteins (IRP)
• The RNA binding of IRPs is dependent on iron levels
• Thus, iron deprivation leads to increased RNA binding, which masks the recognition site of endonucleases and stabilizes transferrin receptor mRNA
• Western Blotting