Chk2 Mouse, Unlabeled, Clone: 19, BD

Description

• The cell cycle is regulated by multiple checkpoints that determine cell fate
• Such checkpoints ensure that DNA replication and chromosomal segregation are completed with high fidelity
• When DNA is damaged, specific kinases and phosphatases, key components of cell cycle checkpoints, function to arrest the cell cycle and provide the necessary time for DNA repair
• For example, DNA damage induces arrest of the cell cycle at the G2 checkpoint via Wee1-mediated inhibitory phosphorylation of the kinase Cdc2
• Inhibition is relieved by the Cdc25C phosphatase via Cdc2 dephosphorylation
• In turn, the Chk1 and Chk2 protein kinases phosphorylate and inhibit Cdc25C, thus preventing activation of the Cdc2-cyclin B complex and entry into mitosis
• Chk2, the human homolog of S
• cerevisiae Rad53 and S
• pombe Cds1, contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA) which is found in other cell cycle kinases
• Chk2 is expressed during late G1 to M phase and is found in the nucleoplasm
• Thus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage
• Host Species: Mouse Clone: 19 Isotype: IgG1 Species Reactivity: Mouse Immunogen: Mouse Chk2 aa
• 31-234 Formula Weight [Chemical]: 60kDa Immunofluorescence, Western Blotting