TAP Mouse, Unlabeled, Clone: 19, BD

Description

• Nuclear export of cellular mRNAs is highly selective, wherein only fully processed RNAs are exported
• Retroviral replication requires export of unspliced viral RNAs, which serve as templates for structural proteins and as genomic RNA for progeny virus
• The export of unspliced viral RNA depends on the interaction of cis-acting RNA elements with cellular factors
• In simian type D retroviruses, the cis-acting element is the constitutive transport element (CTE)
• CTE-dependent nuclear transport is mediated by the cellular TAP protein
• TAP (Tip associating protein) is the vertebrate homolog of the yeast nuclear export protein Mex67p
• TAP is a nuclear protein that shuttles between the nucleus and cytoplasm
• It contains an N-terminal nuclear localization and export region (NLS-NES), a central RNA binding domain (RBD), and a C-terminal portion that is required for nuclear localization and nuclear rim association
• The first 372 aa of TAP are necessary and sufficient for binding and nuclear export of CTE-containing RNA
• Thus, TAP contains a novel RNA-binding motif that recognizes CTE-containing RNA and interacts with other components of the nuclear transport machinery.