Bub3 Mouse, Unlabeled, Clone: 31, BD

Description

• Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately attached to mitotic spindles before the onset of anaphase
• Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed
• In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores
• In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity
• Bub3 contains four WD repeats, three in the N-terminus and one in the C-terminus, and a central Bub1-binding domain
• During prophase and prometaphase, Bub3 localizes to the kinetochore before attachment to microtubules
• In addition, taxol-induced formation of lagging chromosomes due to a delay of cell cycle progression increases the level of Bub3 co-localized with kinetochores, while correctly aligned chromosomes found in metaphase do not exhibit this co-localization
• Thus, Bub3, in association with Bub1, may be important for sensing kinetochore attachment to microtubules during the prometaphase to metaphase transition
• Host Species: Mouse Clone: 31 Isotype: IgG1 Species Reactivity [for Features Main]: Human Immunogen: Human Bub3 aa
• 4-16 Bioimaging, Immunofluorescence, Western Blotting