PNUTS Mouse, Unlabeled, Clone: 47, BD

Description

• Many aspects of cellular physiology are influenced by the serine/threonine protein phosphatase1 (PP1)
• It is a highly conserved eukaryotic protein that reverses the action of protein kinases
• PP1 is key to such processes as cell division, neuronal metabolism, and protein synthesis
• It consists of a catalytic subunit, with a broad substrate specificity, whose action is directed by its association with a family of regulatory proteins including inhibitor-1, inhibitor-2, and NIPP-1
• In response to extracellular stimuli, these proteins interact with PP1 and inhibit its catalytic activity
• Other regulatory proteins are referred to as targeting subunits because they direct PP1 to specific subcellular locations and modulate its activity
• In the cell nucleus, PP1 has been implicated in a variety of processes
• Nuclear PP1 exists in high MW complexes with other proteins including NIPP-1
• PNUTS (phosphatase 1 nuclear targeting subunit) is a ubiquitously expressed nuclear protein that forms a stable complex with PP1 and is thought to modulate its catalytic activity
• Thus, PNUTS is a specific regulatory protein that directs the nuclear function of PP1
• Immunofluorescence, Western Blotting