Eps15 Mouse, Unlabeled, Clone: 17, BD

Description

• The eps15 (EGF receptor pathway substrate clone number 15) gene was isolated using an expression cloning method designed for direct isolation of cDNAs encoding substrates of tyrosine kinases
• The eps15 gene encodes a 145kDa protein that is tyrosine phosphorylated following EGF receptor activation and this receptor directly phosphorylates purified Eps15 in vitro
• Phosphorylation of Eps15 is much more efficient following EGF receptor activation versus erbB2 kinase activation, suggesting the protein is predominantly a part of an EGF receptor signaling pathway
• Overexpression of Eps15 causes transformation of NIH 3T3 cells, implying Eps15 may be involved in the control of cell proliferation
• Eps15 binds to the SH3 domain of Crk through its proline-rich domain at the C-terminal region
• Adaptin α binds to the C-terminus of Eps15 at three different sites ranging from residues 650 to 730
• Eps15, Adaptin α and EGF receptor are co-localized at the clathrin-coated pits after receptor activation
• Therefore, Eps15 may recruit EGF receptors to the clathrin-coated pits for internalization of the activated receptor
• Immunofluorescence, Immunoprecipitation, Western Blotting