Flotillin-1 Mouse, Unlabeled, Clone: 18, BD

Description

• Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes
• These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors
• Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations
• Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae
• Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant
• Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae
• Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCα, Ras, Rap Src-like kinases, Gαβγ,and GPI-linked receptors
• Immunofluorescence, Immunoprecipitation, Western Blotting