p38 MAPK (pT180/pY182) Mouse, Unlabeled, Clone: 30, BD

Description

• Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS)
• Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-α
• LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs)
• MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death
• The p38 MAP kinases include p38α, β, γ, and δ
• These ser/thr kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII
• Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3, MKK4, and MKK6
• This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes
• Thus, p38 MAPKs are central kinases in muliple signal transduction pathways
• Immunofluorescence, Intracellular Staining, Western Blotting