Prostasin Mouse anti-Human, Unlabeled, Clone: 2, BD

Description

• Prostasin is a serine protease expressed as both GPI-anchored and secreted forms
• Prostasin displays trypsin-like enzymatic activity by hydrolyzing substrates, such as D-Pro-Phe-Arg-AMC
• This activity is inhibited by aprotinin, antipain, leupeptin, and benzamidine
• The highest levels of prostasin protein are found in semen and the prostate, but many other tissues express prostasin at low levels
• The sequence of prostasin is similar to other proteases, such as prostase, testisin, plasma kallikrein, and acrosin
• In seminal fluid, prostasin can be found in complex with prostasin-binding protein, which can inhibit prostasin enzymatic activity
• Prostasin mRNA is found in normal prostate epithelial cells and non-invasive prostate cancer cells, but is not expressed in invasive prostate cancer cell lines
• Expression of prostasin in the invasive prostate cancer cell lines, DU-145 and PC-3, reduces the invasiveness of the cells in vitro
• Thus, prostasin is a serine protease that may have roles in normal prostate function and in suppression of tumor cell invasion
• Western Blotting