VCP (Valosin Containing Protein) Mouse, Unlabeled, Clone: 18, BD

Description

• ATPases that belong to the ATPase associated with different cellular activities (AAA) family are homo-oligomeric proteins that have roles in cell cycle regulation, protein degradation, organelle biogenesis, and vesicle mediated-protein transport
• Valosin containing protein (VCP) is a AAA family member that is found as a hexamer in rat liver
• In vitro, VCP catalyzes the hydrolysis of ATP, but this activity is distinct from classical transport ATPases
• VCP protein is found primarily in the transitional elements between the rough and smooth ER (TER), but stimulation with EGF leads to VCP translocation to the nucleus
• Antibodies to VCP can perturb cell-free formation of transition vesicles from isolated TER of rat liver, and can inhibit transfer of material from ER to Golgi in a reconstituted membrane system
• VCP is phosphorylated after T-cell activation, and PTPH1 dephosphorylation of VCP correlates with suppression of cell growth
• In addition, the N-terminal region of VCP binds to the DNA damage repair protein, BRCA1
• Thus, VCP may have roles in both ER protein transport and nuclear function, which are important for cell growth and survival
• Immunofluorescence, Western Blotting