Cathespin D Mouse anti-Human, Unlabeled, Clone: 49, BD

Description

• Cathepsin D, an enzyme that degrades proteins, was originally cloned during the search of estrogen responsive genes in MCF-7 cells
• Cathepsin D is synthesized as the 43kDa preprocathepsin D that is cleaved to form a 46kDa glycosylated procathepsin D
• Procathepsin is then processed into a 44kDa active Cathepsin D
• The active and mature forms undergo a further cleavage that yields 28kDa and 15kDa (heavy and light chains, respectively) fragments in SDS-PAGE
• The heavy and light chains of Cathepsin D are released into the extracellular medium
• The maturation process of Cathepsin D occurs through the transit from the endoplasmic reticulum, Golgi apparatus, and to the lysosomes
• Estrogens stimulate cell proliferation in a number of tumor cell lines and anti-estrogen therapy is often used in the treatment of breast cancer patients
• Therefore, Cathepsin D, which is estrogen inducible, may have a role during the pathogenesis of breast tumors
• Additionally, several other roles have been proposed for this enzyme, such as tissue remodeling, tumor invasion, and embryo implantation
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting