IgD Mouse anti-Human, Brilliant Ultraviolet 737, Clone: IA6-2, BD Horizon™

Description

• The IA6-2 monoclonal antibody specifically binds to the heavy chain of human Immunoglobulin D (IgD)
• IgD is a member of the immunoglobulin superfamily that exists in type 1-membrane (mIgD) and soluble glycoprotein forms
• mIgD is expressed on mature naοve B cells (along with membrane IgM) and serves as a B-cell receptor for antigen (BCR)
• In response to antigen binding, the mIgD BCR, in association with other signaling molecules including CD79a and CD79b, can transduce activating or tolerizing signals intracellularly into B lymphocytes
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).