CD11b Rat anti-Mouse, Brilliant Ultraviolet 737, Clone: M1/70, BD Horizon™

Description

• The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM)
• CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family
• CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3)
• Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi
• Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes
• Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface
• The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis
• Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different BUV737 reagents (e.g., CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.