CD31 Rat anti-Murine, Brilliant Ultraviolet 737, Clone: MEC 13.3, BD Horizon™

Description

• The MEC13.3 antibody reacts with CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1)
• CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion
• CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is also expressed on many peripheral leukocytes and platelets
• It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells
• CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues
• Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in the regulation of ligand specificity
• CD38 and vitronectin receptor ( α v β 3 integrin, CD51/CD61) are proposed to be ligands for CD31
• CD31-mediated endothelial cell-cell interactions are involved in angiogenesis
• The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different BUV737 reagents (e.g., CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.