CD195 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: 2D7/CCR5, BD Horizon™

Description

• The 2D7/CCR5 monoclonal antibody specifically binds to the human chemokine receptor, CCR5
• CCR5 is also known as CD195
• CCR5 is a seven transmembrane-spanning G protein-associated molecule
• CCR5 belongs to the beta chemokine receptor family
• It is expressed on a subset of T lymphocytes (CD3+CD45RO+CD95+)
• CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation
• It signals a response to at least three chemokines: Regulated upon Activation Normal T-cell Expressed and Secreted (RANTES), Macrophage Inflammatory Protein-1 (MIP-1) and Monocyte Chemoattractant Protein 2 (MCP-2)
• Additionally, CCR5 has been found to be a coreceptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission
• Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection
• This antibody has been shown to block ligand and gp120 binding
• It is also able to neutralize HIV infection
• The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).