Pax-5 Mouse, Unlabeled, Clone: 24, BD

Description

• There are at least nine members of the paired box (Pax) gene family whose protein products are transcription factors involved in development
• The conserved paired box DNA-binding domain is found in the N-terminal region of Pax proteins
• An octomer and homeodomain sequence are conserved in the center of the proteins
• The Ser/Thr/Pro-rich region in the C-terminal portion contains a conserved 100 amino acid transactivating domain
• One of the best studied Pax family members, Pax 5, is a B cell specific activator protein (BSAP)
• In the early stages of B cell development, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20
• Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells
• Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis
• Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis
• Pax-5 has been found to be important in both B cell and nervous system development
• Immunofluorescence, Immunoprecipitation, Western Blotting