Ki-67 Mouse anti-Human, Unlabeled, Clone: 35, BD

Description

• Cellular proliferation is a complex multi-faceted process, central to biological events ranging from embryonic development to wound healing
• Although proliferation is tightly controlled, dysregulation often results in tumorigenesis
• The regulatory process involves the expression of many cell-cycle-associated proteins that are subject to cycle-dependent modification
• Such protein expression may be monitored to assess the proliferative capacity of cells, especially tumorigenic cells.The Ki-67 antigen is a nuclear protein expressed exclusively in proliferating cells during all active parts of the cell cycle
• However, it is absent in quiescent cells and during DNA repair
• The distribution of Ki-67 changes during different stages of the cell cycle
• During G1, it is localized to the perinuclear region, but is primarily found in the nuclear matrix in later phases
• During mitosis, it associates with the condensed chromosomes
• The nuclear localization of Ki-67 and strict association with the cell cycle indicate its importance in the regulation of cell division
• Therefore, Ki-67 has become an important marker of proliferating cells, and may also be a marker for disitinct nuclear matrix compartments
• Immunofluorescence, Western Blotting