Stat3 (pY705) Mouse, Unlabeled, Clone: 4/P-STAT3, BD

Description

• Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors
• Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation
• Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes
• Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner
• Stat3 is a 92kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF
• Stat3 activation occurs via tyrosine phosphorylation at Y705
• Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1
• Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression
• It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1
• In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway
• Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter
• This site is similar to the GAS element that is present in IFN-γ induced genes
• Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity
• Intracellular Staining, Western Blotting