Stat3 (pS727) Mouse, Unlabeled, Clone: 49/P-STAT3, BD

Description

• The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription
• Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6
• Stat3 is a 92kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF
• Stat3 is phophorylated at serine 727 (S727) via the MAPK pathway
• The S727 residue is located at a conserved Pro-X-Ser-Pro sequence, which is recognized by the protein kinase ERK
• Activation through the S727 residue is thought to lead to initiation of transcription
• Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression
• It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1
• In addition to serine phosphorylation, Stat3 is also phosphorylated at tyrosine 705 by JAK1 in response to cytokine stimulation
• Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter
• This site is similar to the GAS element that is present in IFN-g-induced genes
• Thus, phosphorylation of S727 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity
• Immunofluorescence, Western Blotting