CD56 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: NCAM16.2 (also known as NCAM 16), BD Horizon™

Description

• The NCAM16.2 monoclonal antibody specifically binds to human CD56
• It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39
• The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes
• It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes
• CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity
• CD56 antigen density on NK lymphocytes increases upon cellular activation
• The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis
• CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).