CD56 Mouse anti-Human, Brilliant Ultraviolet 563, Clone: NCAM16.2, BD Horizon™

Description

• The NCAM16.2 monoclonal antibody specifically binds to human CD56
• It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39
• The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes
• It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes
• CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity
• CD56 antigen density on NK lymphocytes increases upon cellular activation
• The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis
• CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells
• The antibody was conjugated to BD Horizon BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348nm and an acceptor dye
• The tandem has an Em Max at 563 nm
• BD Horizon BUV563 can be excited by the 355nm ultraviolet laser
• On instruments with a 561nm Yellow-Green laser, the recommended bandpass filter is 585/15nm with a 535nm long pass to minimize laser light leakage
• When BD Horizon BUV563 is used with an instrument that does not have a 561nm laser, a 560/40nm filter with a 535nm long pass may be more optimal
• Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.