CD45 Mouse, Brilliant Violet 421, Clone: D058-1283, BD Optibuild™

Description

• D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes
• It was developed using Rhesus peripheral whole blood as the immunogen
• It does not cross-react with human leucocytes
• This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells
• Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples
• This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes
• In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.