CD325 Mouse anti-Human, BUV395, Clone: 8C11, BD Optibuild™

Description

• The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325)
• Cadherins are a family of Ca2+ -dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during d evelopment
• Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ)
• Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin) , R-cadherin, cadherin 5, L-CAM, and EP-cadherin
• N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver
• Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development
• In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases
• Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin
• However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association
• Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphoryla tion/dephosphorylation events
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.