CD325 Mouse anti-Human, Brilliant Violet 650, Clone: 8C11, BD Optibuild™

Description

• The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325)
• Cadherins are a family of Ca2+ -dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during d evelopment
• Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ)
• Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin) , R-cadherin, cadherin 5, L-CAM, and EP-cadherin
• N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver
• Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development
• In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases
• Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin
• However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association
• Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphoryla tion/dephosphorylation events
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.