CD83 Mouse anti-Human, Brilliant Violet 650, Clone: HB15e, BD Optibuild™

Description

• The HB15e monoclonal antibody specifically binds to a 45 kDa type 1 transmembrane glycoprotein member of the Ig superfamily
• CD83 is composed of a single V-type Ig extracellular domain with a C-terminal cytoplasmic tail
• Cell surface CD83 is expressed mainly by follicular dendritic cells, circulating dendritic cells, interdigitating dendritic cells in lymphoid tissues, in vitro-generated dendritic cells and thymic dendritic cells
• However, its expression is not restricted to dendritic cells
• CD83 is also expressed on some germinal center B cells and some lymphoblastoid cell lines
• Although its function is not known, it may play a role in cell-cell interaction during antigen presentation
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.