CD183 Mouse anti-Human, Brilliant Violet 650, Clone: 1C6/CXCR3, BD Optibuild™

Description

• The 1C6/CXCR3 monoclonal antibody specifically binds to human CD183, also known as the CXCR3 chemokine receptor
• CD183 is a 40-41 kDa seven-transmembrane protein and member of the G protein-coupled receptor family
• CD183 is expressed primarily on activated T cells that infiltrate inflammatory sites
• It has also been detected on some circulating T cells, B cells, and NK cells
• Reports show that some CXCR3-positive T cells also express CCR5 and are mostly CD45RO-positive cells
• Three ligands for CXCR3 have been identified
• They are CXCL9 (Mig/monokine induced b y interferon-γ), CXCL10 (IP-10/interferon-γinducible 10- kD protein), and CXCL11 (I-TAC/interferon-inducible T-cell alpha chemoattractant)
• These chemokines are produced by a variety of cells upon stimulation by IFN-γand interact with CXCR3 to medi ate T-cell chemotaxis
• This reagent has been reported to be suitable for immunohistochemical staining of acetone-fixed, frozen sections and/or formalin-fixed, paraffin-embedded tissue sections with citrate pretreatment
• Clone 1C6/CXCR3 also cross reacts with a subset of peripheral blood lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys
• The distribution of lymphocytes is similar to that observed with CD183-positive peripheral blood lymphocytes from normal human donors
• CXCR3 has been clustered as CD183 in the VIIth HLDA workshop
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.