CD184 Rat anti-Mouse, Brilliant Violet 605, Clone: 2B11/CXCR4, BD Optibuild™

Description

• The 2B11/CXCR4 monoclonal antibody specifically binds to mouse CD184, which is also known as the C-X-C Chemokine Receptor type 4 , CXCR4
• CXCR4 (previously known as Fusin and LESTR), is a seven-transmembrane, G-protein-coupled receptor
• It is the specific receptor for the CXC chemokine, SDF-1/CXCL12
• Mouse CXCR4 shows 91% homology at the amino acid level with human CXCR4
• CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes
• Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry
• The mouse Cxcr4 gene has been mapped to chromosome 1
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.