CD183 Mouse anti-Human, Brilliant Violet 510, Clone: 1C6/CXCR3, BD Optibuild™

Description

• The 1C6/CXCR3 monoclonal antibody specifically binds to human CD183, also known as the CXCR3 chemokine receptor
• CD183 is a 40-41 kDa seven-transmembrane protein and member of the G protein-coupled receptor family
• CD183 is expressed primarily on activated T cells that infiltrate inflammatory sites
• It has also been detected on some circulating T cells, B cells, and NK cells
• Reports show that some CXCR3-positive T cells also express CCR5 and are mostly CD45RO-positive cells
• Three ligands for CXCR3 have been identified
• They are CXCL9 (Mig/monokine induced b y interferon-γ), CXCL10 (IP-10/interferon-γinducible 10- kD protein), and CXCL11 (I-TAC/interferon-inducible T-cell alpha chemoattractant)
• These chemokines are produced by a variety of cells upon stimulation by IFN-γand interact with CXCR3 to medi ate T-cell chemotaxis
• This reagent has been reported to be suitable for immunohistochemical staining of acetone-fixed, frozen sections and/or formalin-fixed, paraffin-embedded tissue sections with citrate pretreatment
• Clone 1C6/CXCR3 also cross reacts with a subset of peripheral blood lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys
• The distribution of lymphocytes is similar to that observed with CD183-positive peripheral blood lymphocytes from normal human donors
• CXCR3 has been clustered as CD183 in the VIIth HLDA workshop
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.