CD146 Rat anti-Mouse, Brilliant Violet 650, Clone: ME-9F1, BD Optibuild™

Description

• The ME-9F1 monoclonal antibody specifically binds to mouse CD146
• The CD146 adhesion molecule is a type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily
• CD146 is expressed by blood vessel endothelial cells and may play roles in forming intercellular junctions between endothelial cells and influencing the transendothelial migration of other cell types
• CD146 is also expressed by some melanoma cell lines, NK cells and neutrophils
• CD146 is not detectable on mouse monocytes, dendritic cells, T cells, NKT cells, B cells and smooth muscle cells
• Increased expression of CD146 is reportedly associated with NK cell maturation and may be used to characterize different functional NK cell subsets
• Activated CD146-positive mouse NK cells reportedly are less cytotoxic and secrete less IFN- γthan their CD146-negative counterparts
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.