CD276 Rat anti-Mouse, Brilliant Violet 650, Clone: MIH32, BD Optibuild™

Description

• The MIH32 monoclonal antibody specifically binds to CD276, also known as B7-H3 (B7 homolog 3)
• CD276 is a type I transmembrane glycoprotein and member of the B7-family of regulatory proteins
• The expression of B7-H3 can be induced on T cells, natural killer (NK) cells and antigen presenting cells
• B7-H3 is up-regulated during the differentiation of monocytes into dendritic cells or during the interaction between dendritic cells and regulatory T cells
• In addition, B7-H3 is found to be expressed on fibroblasts, fibroblast-like synoviocytes and epithelial cells
• CD276 (B7-H3) can function as a positive or a negative regulator of T responses
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.