CD102 Rat anti-Mouse, Brilliant Violet 605, Clone: 3C4(mIC2/4), BD Optibuild™

Description

• The 3C4 (mIC2/4) monoclonal antibody specifically binds to the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1
• CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls
• It is also expressed on a variety of leukocyte cell lines
• CD102 does not appear to be involved in the development of hematopoietic cells
• In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen
• The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.