CD146 Rat anti-Mouse, Brilliant Violet 605, Clone: ME-9F1, BD Optibuild™

Description

• The ME-9F1 monoclonal antibody specifically binds to mouse CD146
• The CD146 adhesion molecule is a type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily
• CD146 is expressed by blood vessel endothelial cells and may play roles in forming intercellular junctions between endothelial cells and influencing the transendothelial migration of other cell types
• CD146 is also expressed by some melanoma cell lines, NK cells and neutrophils
• CD146 is not detectable on mouse monocytes, dendritic cells, T cells, NKT cells, B cells and smooth muscle cells
• Increased expression of CD146 is reportedly associated with NK cell maturation and may be used to characterize different functional NK cell subsets
• Activated CD146-positive mouse NK cells reportedly are less cytotoxic and secrete less IFN- γthan their CD146-negative counterparts
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.